Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides

FEBS Lett. 2000 Nov 17;485(1):40-6. doi: 10.1016/s0014-5793(00)02181-5.


Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results: carbohydrate structure [see text] Mass spectrometry and serology revealed that the O-chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species.

MeSH terms

  • Antibodies, Monoclonal
  • Bordetella / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chromatography, Thin Layer
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Gas Chromatography-Mass Spectrometry
  • Hydrolysis
  • Lipopolysaccharides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Oligosaccharides / analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


  • Antibodies, Monoclonal
  • Lipopolysaccharides
  • Oligosaccharides