Double Oxidation of D-xylose to D-glycero -pentos-2,3-diulose (2,3-diketo-D-xylose) by Pyranose Dehydrogenase From the Mushroom Agaricus Bisporus

Carbohydr Res. 2000 Oct 20;329(1):219-25. doi: 10.1016/s0008-6215(00)00167-1.

Abstract

Pyranose dehydrogenase purified to homogeneity from the mycelia of the basidiomycete fungus Agaricus bisporus catalyzed the oxidation of D-xylose at C-2 to D-threo-pentos-2-ulose (2-keto-D-xylose) and successively at C-3 to D-glycero-pentos-2,3-diulose (2,3-diketo-D-xylose) using 1,4-benzoquinone as an electron acceptor. The sites of oxidation were deduced from the spectroscopic analysis (MS, NMR) of the N,N-diphenylhydrazone derivatives of the reaction products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricus / enzymology*
  • Carbohydrate Dehydrogenases / isolation & purification
  • Carbohydrate Dehydrogenases / metabolism*
  • Chromatography, High Pressure Liquid
  • Hydrazones / chemistry
  • Ketones / chemistry
  • Ketones / metabolism
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Oxidation-Reduction
  • Xylose / analogs & derivatives
  • Xylose / chemistry
  • Xylose / metabolism*

Substances

  • 2,3-diketoxylose
  • Hydrazones
  • Ketones
  • Xylose
  • Carbohydrate Dehydrogenases