Identification of KLF13 and KLF14 (SP6), novel members of the SP/XKLF transcription factor family

Genomics. 2000 Nov 15;70(1):93-101. doi: 10.1006/geno.2000.6362.


Using the sequence of the SP1 zinc-finger DNA-binding domain as a probe to screen a mouse EST database, we identified two novel members of the SP/XKLF transcription factor family, KLF13 and KLF14. The mouse Klf13 cDNA (1310 bp in length) contains a single open reading frame of 288 amino acids with a DNA-binding domain closely related to that of the human RFLAT-1 protein and a putative transactivator N-terminal domain rich in proline and alanine residues. The mouse Klf13 gene seems to be the homologue of the human RFLAT1 gene. The mouse Klf14 sequence is homologous to a human genomic sequence from chromosome 17 that is believed to code for a protein with three zinc fingers at the end of its C-terminal domain. Using reverse transcription-polymerase chain reaction, we showed ubiquitous expression of Klf13 and Klf14 in adult mice. A third member of this family was also identified in a human EST database; this sequence was found to be identical to KLF11 (TIEG2), recently identified by Cook et al. (1998, J. Biol. Chem. 273: 25929-25936). The corresponding mouse cDNA was isolated and sequenced. The three genes were localized in the human and the rat: chromosomes 15 (human KLF13), 17q21.3-q22 (human KLF14; HGMW-approved symbol SP6), and 2p25 (human KLF11) and chromosomes 1q31-q32 (rat Klf13), 10q31-q32.1 (rat Klf14) (SP6), and 6q16-q21 (rat Klf11).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins
  • Base Sequence
  • Cell Cycle Proteins / genetics
  • Chromosome Mapping
  • Chromosomes, Human, Pair 15
  • Expressed Sequence Tags
  • Humans
  • Kruppel-Like Transcription Factors
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Rats
  • Repressor Proteins / genetics
  • Sequence Homology, Amino Acid
  • Sp Transcription Factors
  • Trans-Activators / classification
  • Trans-Activators / genetics*
  • Transcription Factors / classification
  • Transcription Factors / genetics*
  • Zinc Fingers*


  • Apoptosis Regulatory Proteins
  • Cell Cycle Proteins
  • KLF11 protein, human
  • KLF11 protein, rat
  • KLF13 protein, human
  • KLF14 protein, human
  • Klf13 protein, mouse
  • Kruppel-Like Transcription Factors
  • Repressor Proteins
  • Sp Transcription Factors
  • Sp6 protein, mouse
  • Trans-Activators
  • Transcription Factors

Associated data

  • GENBANK/AJ275987
  • GENBANK/AJ275988
  • GENBANK/AJ275989