Developmental expression of the Arabidopsis thaliana CycA2;1 gene

Planta. 2000 Oct;211(5):623-31. doi: 10.1007/s004250000333.


The associations of cyclins with highly conserved cyclin-dependent kinases are key events in the regulation of cell cycle progression. The spatio-temporal expression of an Arabidopsis thaliana (L.) Heynh. mitotic cyclin, Arath;CycA2;1, was studied by histochemical beta-glucuronidase (GUS) analysis and in-situ hybridizations. The CycA2,1] promoter was active in the egg apparatus before fertilization. During embryogenesis, CycA2;1:gus expression was found in the embryo and the developing endosperm. Throughout plant development, CycA2;1 transcripts were found in both dividing and non-dividing cells, indicating that the expression of this cyclin is not a limiting factor for cell division. In the pericycle and stelar parenchyma, CycA2;1 transcripts were located at the xylem poles, a position that can be correlated with competence for lateral root formation. In addition, CycA2;1:gus expression was upregulated in roots by auxins and in the shoot apex by cytokinins. Transcription of CycA2;1 was shown by reverse transcription-polymerase chain reaction to be strongly induced by sucrose in A. thaliana cell suspensions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Cell Cycle / physiology*
  • Cells, Cultured
  • Cyclins / genetics*
  • Cyclins / metabolism
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant*
  • Genes, Reporter
  • Glucuronidase / genetics
  • Plants, Genetically Modified
  • Promoter Regions, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seeds / physiology


  • Cyclins
  • Glucuronidase