A transcription reinitiation intermediate that is stabilized by activator

Nature. 2000 Nov 9;408(6809):225-9. doi: 10.1038/35041603.

Abstract

High levels of gene transcription by RNA polymerase II depend on high rates of transcription initiation and reinitiation. Initiation requires recruitment of the complete transcription machinery to a promoter, a process facilitated by activators and chromatin remodelling factors. Reinitiation probably occurs through a different pathway. After initiation, a subset of the transcription machinery remains at the promoter, forming a platform for assembly of a second transcription complex. Here we describe the isolation of a reinitiation intermediate that includes transcription factors TFIID, TFIIA, TFIIH, TFIIE and Mediator. This intermediate can act as a scaffold for formation of a functional reinitiation complex. Formation of this scaffold is dependent on ATP and TFIIH. The scaffold is stabilized in the presence of the activator Gal4-VP16, but not Gal4-AH, suggesting a new role for some activators and Mediator in promoting high levels of transcription.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins
  • Fungal Proteins / metabolism
  • Macromolecular Substances
  • Promoter Regions, Genetic
  • RNA Polymerase II / chemistry
  • RNA Polymerase II / metabolism
  • RNA, Fungal / metabolism
  • Saccharomyces cerevisiae Proteins*
  • TATA-Binding Protein Associated Factors*
  • Trans-Activators / metabolism
  • Transcription Factor TFIIA
  • Transcription Factor TFIID*
  • Transcription Factor TFIIH
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism*
  • Transcription Factors, TFII*
  • Transcription, Genetic*
  • Yeasts / genetics

Substances

  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Gal-VP16
  • Macromolecular Substances
  • RNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • TAF6 protein, S cerevisiae
  • TATA-Binding Protein Associated Factors
  • Trans-Activators
  • Transcription Factor TFIIA
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription Factors, TFII
  • transcription factor TFIIE
  • Transcription Factor TFIIH
  • Adenosine Triphosphate
  • RNA Polymerase II