MHC class II and c-kit expression allows rapid enrichment of T-cell progenitors from total bone marrow cells

Blood. 2000 Dec 1;96(12):3988-90.

Abstract

T-cell progenitors in the embryonic bone marrow express the tyrosine kinase receptor c-kit. RR5, an anti-MHC class II beta chain monoclonal antibody, subdivides this c-kit positive population. Intrathymic transfer experiments showed that most of the T-cell progenitors belong to the MHC class II(+)/c-kit(+) bone marrow population in the embryo and young adult. On transplantation, these bone marrow progenitors lose this expression and differentiate into CD4 CD8 T lymphocytes. In contrast, erythroid progenitors are restricted to the MHC class II(-)/c-kit(+) population. The MHC class II(+)/c-kit(+) pro-T cells are metabolically active, because they stain brightly with rhodamin 123. Their cyclin A and B expression level suggests that they are in the mitotic phase of the cell cycle. Thus, we define an easy sorting protocol, which allows enrichment of T-cell progenitors from total bone marrow hemopoietic cells. (Blood. 2000;96:3988-3990)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology
  • Cell Differentiation / immunology
  • Cell Separation / methods
  • Chickens
  • Embryo, Nonmammalian
  • Erythroid Precursor Cells / cytology
  • Erythroid Precursor Cells / immunology
  • Flow Cytometry
  • Genes, MHC Class II / immunology*
  • Proto-Oncogene Proteins c-kit / immunology*
  • Stem Cells / cytology
  • Stem Cells / immunology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology

Substances

  • Proto-Oncogene Proteins c-kit