Bombesin-like peptide and receptors in lung injury models: diverse gene expression, similar function

Peptides. 2000 Nov;21(11):1627-38. doi: 10.1016/s0196-9781(00)00294-1.


We previously demonstrated that bombesin-like peptide (BLP) mediates lung injury in premature infants with bronchopulmonary dysplasia (BPD). We now investigate gene expression and function of BLP (gastrin-releasing peptide, GRP) and BLP-receptors (GRP-R and BRS-3) in lung from two baboon BPD models. In the "interrupted gestation model," only GRP mRNA was up-regulated. In the "hyperoxic model," GRP-R mRNA was up-regulated. In lung explants from O2-treated animals, all BPD animals responded to 1nM bombesin, whereas non-BPD animals did not; the opposite effect was observed with a BLP blocking antibody. Cumulatively, these observations suggest that novel BLPs and/or BLP receptors are likely to be implicated in the pathogenesis of BPD.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bombesin / metabolism
  • Bombesin / pharmacology
  • Dexamethasone / pharmacology
  • Gastrin-Releasing Peptide / biosynthesis*
  • Gastrin-Releasing Peptide / physiology*
  • Gene Expression
  • In Situ Hybridization
  • Lung / embryology
  • Lung / metabolism*
  • Organ Culture Techniques
  • Oxygen / metabolism
  • Papio / metabolism*
  • Phosphatidylcholines / pharmacology
  • RNA, Messenger / metabolism
  • Receptors, Bombesin / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Up-Regulation*


  • Phosphatidylcholines
  • RNA, Messenger
  • Receptors, Bombesin
  • bombesin receptor subtype 3
  • di-(8-methylstearoyl)phosphatidylcholine
  • Dexamethasone
  • Gastrin-Releasing Peptide
  • Bombesin
  • Oxygen