Expression cloning of Xantivin, a Xenopus lefty/antivin-related gene, involved in the regulation of activin signaling during mesoderm induction

Mech Dev. 2000 Dec;99(1-2):3-14. doi: 10.1016/s0925-4773(00)00465-2.

Abstract

In a screening for activin-responsive genes, we isolated a Xenopus lefty/antivin-related gene, called Xantivin (Xatv). In the animal cap assay, the expression of Xatv was induced by activin signaling, and in the embryo, by nodal-related genes. Overexpression of Xatv in the marginal zone caused suppression of mesoderm formation and gastrulation defects, and inhibited the secondary axis formation induced by Xnr1 and Xactivin, suggesting that Xatv acted as a feedback inhibitor of activin signaling. However, in the animal cap, Xatv failed to antagonize Xnr1 and Xactivin. This result suggested that Xatv has different responses in the marginal zone and in the animal region, and antagonizes to a higher degree activin signaling in the marginal zone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activins
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Cycloheximide / pharmacology
  • DNA, Complementary / metabolism
  • Embryo, Nonmammalian / metabolism
  • Gastrula / metabolism
  • Gene Library
  • Humans
  • In Situ Hybridization
  • Inhibins / metabolism*
  • Left-Right Determination Factors
  • Mesoderm / metabolism*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Protein Synthesis Inhibitors / pharmacology
  • RNA, Messenger / metabolism
  • Recombinant Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Signal Transduction*
  • Time Factors
  • Transforming Growth Factor beta / biosynthesis*
  • Transforming Growth Factor beta / genetics*
  • Transforming Growth Factor beta / metabolism*
  • Xenopus / embryology*
  • Xenopus Proteins*

Substances

  • DNA, Complementary
  • LEFTY1 protein, human
  • Left-Right Determination Factors
  • Protein Synthesis Inhibitors
  • RNA, Messenger
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Xenopus Proteins
  • lefty protein, Xenopus
  • Activins
  • Inhibins
  • Cycloheximide