Enhanced production of IL-8 in chronic but not in early ileal lesions of Crohn's disease (CD)

Clin Exp Immunol. 2000 Nov;122(2):180-5. doi: 10.1046/j.1365-2249.2000.01364.x.


Distinct Th1/Th2 patterns have been observed during the evolution of CD. The aim of this study was to compare neutrophil involvement and IL-8 mRNA and protein expression during early recurrent lesions and chronic phases of CD. Twenty-nine patients with CD having ileocolonic resection with anastomosis were studied. Biopsies were obtained during surgery from the non-inflamed ileal mucosa and from chronic ileal lesions. Endoscopic ileal biopsies were also taken from early recurrent ileal lesions occurring 3 months after surgery. Neutrophil counts were performed and mucosal IL-8 levels were evaluated by competitive reverse transcriptase-polymerase chain reaction and immunohistochemistry. Early recurrent ileal lesions were characterized by low neutrophil counts and IL-8 production at the mRNA and protein levels compared with the ileal chronic lesions. The main cellular sources of IL-8 in the early recurrent lesions were neutrophils, while in chronic lesions the majority of IL-8-stained cells were CD3+ T cells and macrophages. These results confirmed that the nature of the inflammatory infiltrate and the expression of cytokine profiles may differ between the acute and chronic phases of CD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Chronic Disease
  • Crohn Disease / etiology
  • Crohn Disease / immunology*
  • Crohn Disease / pathology
  • DNA Primers / genetics
  • Female
  • Humans
  • Ileum / immunology
  • Ileum / pathology
  • Interleukin-8 / biosynthesis*
  • Interleukin-8 / genetics
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / pathology
  • Male
  • Neutrophils / immunology
  • Neutrophils / pathology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Time Factors


  • DNA Primers
  • Interleukin-8
  • RNA, Messenger