Enhancement of OspC expression by Borrelia burgdorferi in the presence of tick hemolymph

FEMS Microbiol Lett. 2000 Dec 1;193(1):137-41. doi: 10.1111/j.1574-6968.2000.tb09415.x.


Borrelia burgdorferi B31 cultured at 28 degrees C was used to assay the effects of tick hemolymph on outer surface protein C (OspC) surface expression. Using immunofluorescence, OspC surface expression was shown to increase in cultures treated with hemolymph from both Ixodes scapularis and Dermacentor variabilis, while control samples (non-hemolymph treated) showed no increase in the levels of OspC. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis also showed an increase in OspC from spirochetes exposed to tick hemolymph. In addition, a lower-molecular-mass protein band of unknown identity was also enhanced in the hemolymph-exposed spirochetes. The significance of these effects of tick hemolymph on B. burgdorferi outer surface protein expression is unknown.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial*
  • Bacterial Outer Membrane Proteins / metabolism*
  • Borrelia burgdorferi Group / metabolism*
  • Dermacentor*
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Hemolymph*
  • Ixodes*
  • Microscopy, Fluorescence
  • Species Specificity
  • Temperature


  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • OspC protein