Analysis of cepA and other Bacteroides fragilis genes reveals a unique promoter structure

FEMS Microbiol Lett. 2000 Dec 1;193(1):149-54. doi: 10.1111/j.1574-6968.2000.tb09417.x.


There is little known about the sequences that mediate the initiation of transcription in Bacteroides fragilis, thus transcriptional start sites for 13 new genes were determined and a total of 23 promoter regions upstream of the start sites were aligned and similarities were noted. A region at about -7 contained a consensus sequence of TAnnTTTG and upstream in the region centered at about -33, another TTTG motif was found in the majority of promoters examined. Canonical, Escherichia coli, -10 and -35 consensus sequences were not readily apparent. Mutations within the -7 motif indicated the TTTG residues were essential since changes in this sequence reduced the promoter activity to that of a no promoter control in a chloramphenicol acetyl transferase transcriptional fusion model system. Additional fusion studies indicated that the -33 region was also necessary for full activity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Artificial Gene Fusion
  • Bacteroides fragilis / genetics*
  • Base Sequence
  • Chloramphenicol O-Acetyltransferase / genetics
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Mutation
  • Promoter Regions, Genetic*
  • Sequence Alignment
  • beta-Lactamases / genetics*


  • DNA, Bacterial
  • Chloramphenicol O-Acetyltransferase
  • beta-Lactamases