What you should know about PR3-ANCA. Structural aspects of antibodies to proteinase 3 (PR3)

Arthritis Res. 2000;2(4):255-9. doi: 10.1186/ar97. Epub 2000 Jun 12.


Reactive antigenic epitopes on presumed autoantigens of biologic interest have been examined by many researchers. The central third complementarity-determining region (CDR3) residues of a human monoclonal anti-proteinase 3 (PR3) antibody contained many negatively charged aspartic acid residues, perhaps contributing to its reactivity with positively charged PR3 regions. Examination of four other human monoclonal anti-PR3 antibodies shows a number of negatively charged residues within their CDR3 regions. Mapping of segments of linear PR3-epitopes reacting with anti-neutrophil cytoplasmic antibodies (ANCA) demonstrated a preliminary estimate of structures contributing to antigenic determinants. T-cell epitopes on PR3 are reported in studies of chronic myeloid leukemia. These T-cell epitopes appear to be human leukocyte antigen (HLA) A2.1 restricted.

Publication types

  • Review

MeSH terms

  • Animals
  • Antibodies, Antineutrophil Cytoplasmic / immunology*
  • Epitopes / immunology
  • Humans
  • Myeloblastin
  • Recombinant Proteins / immunology
  • Serine Endopeptidases / immunology*
  • T-Lymphocytes / immunology


  • Antibodies, Antineutrophil Cytoplasmic
  • Epitopes
  • Recombinant Proteins
  • Serine Endopeptidases
  • Myeloblastin