Interleukin-8 and SDF1-alpha mRNA expression in colonic biopsies from patients with inflammatory bowel disease

Am J Gastroenterol. 2000 Nov;95(11):3157-64. doi: 10.1111/j.1572-0241.2000.03289.x.


Objectives: Interleukin-8 (IL-8) as an alpha-chemokine recruits and activates neutrophils, which are abundant in the intestinal lesions of ulcerative colitis (UC) and Crohn's disease (CD). Stromal cell-derived factor 1 (SDF1-alpha) is a new chemokine that is chemotactic to neutrophils. The aims of this study were to assess the relative expression of SDF1-alpha and IL-8 mRNA in different colonic regions and patients with inflammatory bowel disease with varied degrees of inflammation in the colon.

Methods: Colon biopsy samples were obtained from 19 patients with UC, 12 with CD, and 5 with irritable bowel syndrome (IBS) who underwent colonoscopy. Levels of IL-8 and SDF1-alpha mRNA expression were measured semiquantitatively by reverse-transcription and polymerase chain reaction amplification. The cytokine mRNA levels were corrected for glyceraldelyde-3-phosphate dehydrogenase mRNA expression.

Results: IL-8 mRNA expression was significantly correlated with SDF1-alpha expression in normal biopsies from IBS patients (r = 0.58, p < 0.01). There was no significant difference in cytokine mRNA expression (IL-8 or SDF1-alpha) across different regions of the colon or rectum in uninflamed normal biopsies. The IL-8 mRNA expression ratios in UC (mean +/- SD, 1.03 +/- 0.52) and CD (0.90 +/- 0.38) patients were significantly higher than in IBS (0.52 +/- 0.17) (p < 0.01, p < 0.05, respectively). The SDF1-alpha mRNA expression ratio in UC (0.30 +/- 0.52) was higher than in both CD (0.21 +/- 0.10) and IBS patients (0.22 +/- 0.11) (p < 0.01, <0.05, respectively). A statistically significant correlation was found between the IL-8 mRNA expression and the colonic inflammation in UC patients (r = 0.44, p < 0.05) but not for SDF1-alpha expression in UC patients.

Conclusions: IL-8 but not SDF1-alpha mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-alpha.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Biopsy
  • Case-Control Studies
  • Chemokine CXCL12
  • Chemokines, CXC / biosynthesis*
  • Colitis, Ulcerative / metabolism*
  • Colon / metabolism
  • Colon / pathology
  • Colonic Diseases, Functional / metabolism
  • Crohn Disease / metabolism*
  • Female
  • Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis
  • Humans
  • Interleukin-8 / biosynthesis*
  • Male
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stromal Cells


  • CXCL12 protein, human
  • Chemokine CXCL12
  • Chemokines, CXC
  • Interleukin-8
  • RNA, Messenger
  • Glyceraldehyde-3-Phosphate Dehydrogenases