Obesity and disturbed lipoprotein profile in estrogen receptor-alpha-deficient male mice

Biochem Biophys Res Commun. 2000 Nov 30;278(3):640-5. doi: 10.1006/bbrc.2000.3827.


Clinical case reports have documented disturbances of carbohydrate and lipid metabolism in aromatase deficient and estrogen resistant males. The aim of the present study was to explore the metabolic functions of estrogens in male mice and to dissect the estrogen receptor (ER) specificity of such effects. Total body fat content and serum levels of leptin were followed in ERalpha knockout (ERKO), ERbeta knockout (BERKO), and ERalpha/beta double knockout (DERKO) mice. Neither the total body fat nor serum leptin levels were altered in any group before or during sexual maturation. However, after sexual maturation ERKO and DERKO, but not BERKO, demonstrated a clear increase in total body fat and enhanced serum leptin levels. Serum cholesterol was increased and a qualitative change in the lipoprotein profile, including smaller LDL particles, was observed in ERKO and DERKO mice. In conclusion, ERalpha but not ERbeta-inactivated male mice develop obesity after sexual maturation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / anatomy & histology
  • Adipose Tissue / physiopathology
  • Animals
  • Cholesterol / blood
  • Crosses, Genetic
  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Fatty Acids, Nonesterified / blood
  • Female
  • Heterozygote
  • Insulin / blood
  • Leptin / blood*
  • Lipoproteins / blood*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Obesity / blood
  • Obesity / genetics*
  • Obesity / physiopathology*
  • Receptors, Estrogen / deficiency
  • Receptors, Estrogen / genetics
  • Receptors, Estrogen / physiology*
  • Sexual Maturation
  • Triglycerides / blood


  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Fatty Acids, Nonesterified
  • Insulin
  • Leptin
  • Lipoproteins
  • Receptors, Estrogen
  • Triglycerides
  • Cholesterol