Electrogenic ion transport in duodenum, an aid in cystic fibrosis diagnosis

Scand J Gastroenterol. 2000 Oct;35(10):1106-9. doi: 10.1080/003655200451252.


Background: Abnormality in chloride transport across epithelial tissues is a basic defect in cystic fibrosis (CF). Our aim was to compare the induced chloride secretion in duodenum in CF patients with different mutations.

Methods: Duodenal biopsies from 9 patients were investigated in a modified Ussing chamber and the secretory response to prostaglandin E2 (PGE2) and acetylcholine (ACh) were measured.

Results: PGE2 and ACh induced no changes in chloride secretion in the AF508 homozygotes. In heterozygotes the induced change in chloride secretion corresponded to the severity of the known mutations.

Conclusion: The secretory response in duodenum in CF is influenced by the patients genotype and mainly related to sweat chloride secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology
  • Adolescent
  • Biopsy
  • Child
  • Child, Preschool
  • Chlorides / metabolism
  • Cystic Fibrosis / diagnosis*
  • Cystic Fibrosis / genetics
  • Dinoprostone / pharmacology
  • Duodenum / metabolism*
  • Epithelium / metabolism
  • Female
  • Heterozygote
  • Homozygote
  • Humans
  • In Vitro Techniques
  • Infant
  • Ion Transport*
  • Male


  • Chlorides
  • Dinoprostone
  • Acetylcholine