Applications of Display Technology in Protein Analysis

Nat Biotechnol. 2000 Dec;18(12):1251-6. doi: 10.1038/82355.

Abstract

Display technology refers to a collection of methods for creating libraries of modularly coded biomolecules that can be screened for desired properties. It has become a routine tool for enriching molecular diversity and producing novel types of proteins. The combination of an ever-increasing variety of libraries of modularly coded protein complexxes with the development of innovative approaches to select a wide array of desired properties has facilitated large-scale analyses of protein-protein/protein-substrate interactions, rapid isolation of antibodies (or antibody mimetics) without immunization, and function-based protein analysis. Several practical and theoretical challenges remain to be addressed before display technology can be readily applied to proteomic studies.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Biotechnology / methods*
  • Humans
  • Peptide Library*
  • Proteins* / genetics
  • Proteins* / metabolism
  • Proteome*

Substances

  • Bacterial Proteins
  • Peptide Library
  • Proteins
  • Proteome