Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein

Cell. 2000 Nov 10;103(4):583-94. doi: 10.1016/s0092-8674(00)00162-8.

Abstract

A broadly conserved membrane-associated protein required for the functional interaction of kinesin-I with axonal cargo was identified. Mutations in sunday driver (syd) and the axonal transport motor kinesin-I cause similar phenotypes in Drosophila, including aberrant accumulations of axonal cargoes. GFP-tagged mammalian SYD localizes to tubulovesicular structures that costain for kinesin-I and a marker of the secretory pathway. Coimmunoprecipitation analysis indicates that mouse SYD forms a complex with kinesin-I in vivo. Yeast two-hybrid analysis and in vitro interaction studies reveal that SYD directly binds kinesin-I via the tetratricopeptide repeat (TPR) domain of kinesin light chain (KLC) with K(d) congruent with 200 nM. We propose that SYD mediates the axonal transport of at least one class of vesicles by interacting directly with KLC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Axonal Transport*
  • Behavior, Animal
  • Biological Transport
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Compartmentation
  • Cloning, Molecular
  • Drosophila / genetics
  • Drosophila Proteins*
  • Insect Proteins / metabolism
  • Kinesin / metabolism*
  • Larva / genetics
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism
  • Molecular Motor Proteins / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Mutation
  • Protein Binding
  • Protein Subunits
  • Two-Hybrid System Techniques
  • trans-Golgi Network / chemistry

Substances

  • Carrier Proteins
  • Drosophila Proteins
  • Insect Proteins
  • Membrane Proteins
  • Microtubule-Associated Proteins
  • Molecular Motor Proteins
  • Protein Subunits
  • syd protein, Drosophila
  • Kinesin

Associated data

  • GENBANK/AF262045
  • GENBANK/AF262046