A chromatin insulator determines the nuclear localization of DNA

Mol Cell. 2000 Nov;6(5):1025-35. doi: 10.1016/s1097-2765(00)00101-5.

Abstract

Chromatin insulators might regulate gene expression by controlling the subnuclear organization of DNA. We found that a DNA sequence normally located inside of the nucleus moved to the periphery when the gypsy insulator was placed within the sequence. The presence of the gypsy insulator also caused two sequences, normally found in different regions of the nucleus, to come together at a single location. Alterations in this subnuclear organization imposed by the gypsy insulator correlated with changes in gene expression that took place during the heat-shock response. These global changes in transcription were accompanied by dramatic alterations in the distribution of insulator proteins and DNA. The results suggest that the nuclear organization imposed by the gypsy insulator on the chromatin fiber is important for gene expression.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Biological Transport
  • Cell Nucleus / chemistry
  • Cell Nucleus / genetics*
  • Cell Nucleus / metabolism*
  • Cell Nucleus Structures / chemistry
  • Cell Nucleus Structures / genetics
  • Cell Nucleus Structures / metabolism
  • Chromatin / chemistry
  • Chromatin / genetics
  • Chromatin / metabolism
  • DNA / chemistry
  • DNA / genetics
  • DNA / metabolism*
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / metabolism
  • Gene Expression Regulation
  • Heat-Shock Response / genetics
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Male
  • Recombinant Proteins / metabolism
  • Regulatory Sequences, Nucleic Acid / genetics*
  • Retroelements / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic / genetics
  • X Chromosome / genetics
  • X Chromosome / metabolism

Substances

  • Chromatin
  • Drosophila Proteins
  • Recombinant Proteins
  • Retroelements
  • Transcription Factors
  • mdg4 protein (gypsy)
  • DNA