Molecular characteristics of eight gastric cancer cell lines established in Japan

Pathol Int. 2000 Oct;50(10):767-77. doi: 10.1046/j.1440-1827.2000.01117.x.

Abstract

Molecular characterization of eight gastric cancer cell lines established in Japan are summarized according to the genetic and epigenetic alterations and growth factor status. TMK-1 poorly differentiated adenocarcinoma cell line harbors mutant p53 tumor suppressor gene and rearrangement of p15MTS2. MKN-1 adenosquamous carcinoma line with mutant p53 reveals silencing of E-cadherin by promoter CpG hypermethylation. MKN-7 well-differentiated adenocarcinoma cell line has amplification of c-erbB2 oncogene and cyclin E gene. MKN-28 well-differentiated adenocarcinoma cell line reveals mutations in p53 and APC tumor suppressor genes and silencing of CD44. The MKN-45 poorly differentiated adenocarcinoma cell line with wild-type p53 is characterized by homozygous deletion of p16CDKN2/MTS1/INK4A and p15MTS2, amplification of c-met oncogene and promoter mutation of E-cadherin. MKN-74 derived from moderately differentiated tubular adenocarcinoma has wild-type p53. KATO-III signet ring cell carcinoma line has genomic deletion of p53, amplification of K-sam and c-met oncogene and mutation of E-cadherin. HSC-39 signet ring cell carcinoma cell line harboring p53 missense mutation has homozygous deletion of p16CDKN2/MTS1/INK4A and p15MTS2, amplifications of c-myc, c-met, K-sam and CD44 gene and mutation in beta-catenin gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Cell Cycle Proteins / genetics
  • Genes, Tumor Suppressor / genetics
  • Growth Substances / genetics
  • Molecular Biology / methods*
  • Oncogenes / genetics
  • Receptors, Growth Factor / genetics
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Tumor Cells, Cultured

Substances

  • Cell Cycle Proteins
  • Growth Substances
  • Receptors, Growth Factor