Characterization of Two Distinct DP-related Genes From Arabidopsis Thaliana

FEBS Lett. 2000 Dec 1;486(1):79-87. doi: 10.1016/s0014-5793(00)02238-9.

Abstract

E2F/DP complexes play a pivotal role in the regulation of the G1/S transition in animals. Recently, plant E2F homologs have been cloned, but DP-related sequences have not been identified so far. Here we report that Arabidopsis thaliana contains at least two different DP-related genes, AtDPa and AtDPb. They exhibit an overall domain organization similar to that of their animal counterparts, although phylogenetic analysis demonstrated that they form a separate subgroup. AtDPs efficiently heterodimerize in vitro with the Arabidopsis E2F-related proteins, AtE2Fa and AtE2Fb through their dimerization domains. AtDPa and AtE2Fa are predominantly produced in actively dividing cells with highest transcript levels in early S phase cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arabidopsis / genetics*
  • Binding Sites
  • Carrier Proteins*
  • Cell Cycle
  • Cell Cycle Proteins*
  • DNA-Binding Proteins*
  • Dimerization
  • E2F Transcription Factors
  • Gene Expression Regulation, Plant
  • Genes, Plant / genetics*
  • Humans
  • Molecular Sequence Data
  • Mutation / genetics
  • Organ Specificity
  • Phylogeny
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Retinoblastoma-Binding Protein 1
  • Sequence Alignment
  • Transcription Factor DP1
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Two-Hybrid System Techniques

Substances

  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • DP transcription factor, plant
  • E2F Transcription Factors
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors

Associated data

  • GENBANK/AJ294531
  • GENBANK/AJ294532
  • GENBANK/AJ294533
  • GENBANK/AJ294534