delta-opioid receptors inhibit neurogenic intestinal secretion evoked by mast cell degranulation and type I hypersensitivity

J Neuroimmunol. 2001 Jan 1;112(1-2):89-96. doi: 10.1016/s0165-5728(00)00387-8.


Histamine and the mast cell degranulator, compound 48/80 produced elevations in short-circuit current, an electrical measure of active anion secretion, across porcine ileal mucosa sheets mounted in Ussing chambers. Luminally-applied beta-lactoglobulin produced similar effects in mucosal sheets from cow's milk-sensitized pigs. Their secretory effects were attenuated by blockers of H(1)-histamine receptors, neuronal conduction or epithelial Na(+)/K(+)/Cl(-) cotransport. The delta-opioid agonist [D-Pen(2), D-Pen(5)]enkephalin suppressed mucosal responses to these substances in a naltrindole-reversible manner. Furthermore, submucosal mast cells and delta-opioid receptor-immunoreactive nerve fibers were observed in close juxtaposition. Intestinal neural pathways linking immediate hypersensitivity to secretory host defense appear to express inhibitory delta-opioid receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • Cell Degranulation*
  • Enkephalin, D-Penicillamine (2,5)- / pharmacology
  • Female
  • Histamine / pharmacology
  • Ileum / metabolism
  • Intestinal Mucosa / metabolism*
  • Male
  • Mast Cells / physiology*
  • Milk Hypersensitivity / metabolism*
  • Receptors, Opioid, delta / physiology*
  • Swine
  • p-Methoxy-N-methylphenethylamine / pharmacology


  • Receptors, Opioid, delta
  • p-Methoxy-N-methylphenethylamine
  • Histamine
  • Enkephalin, D-Penicillamine (2,5)-