5-Hydroxytryptamine(2A) and 5-hydroxytryptamine(1B) receptors are differently affected by the monoamine oxidase A-deficiency in the Tg8 transgenic mouse

Neurosci Lett. 2000 Dec 22;296(2-3):141-4. doi: 10.1016/s0304-3940(00)01653-0.

Abstract

In brainstem-spinal cord preparations of neonatal control C3H and transgenic Tg8 mice where deletion of the gene encoding monoamine oxidase-A results in serotonin (5-hydroxytryptamine (HT)) excess, whole cell recordings of identified phrenic motoneurons (Phr Mns) were performed to study the modulation of their activity by 5-HT. In C3H mice, a dual effect was observed: (i) a facilitation via 5-HT(2A) receptors and (ii) a decrease of the transmission of the central inspiratory drive via 5-HT(1B) receptors. In Tg8 mice, the 5-HT(2A)-mediated facilitation was present but the 5-HT(1B)-mediated decrease was lacking. Therefore, the conservation of the 5-HT(2A) response vs. the loss of the 5-HT(1B) one suggest that the two types of receptors respond differently to 5-HT level changes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / drug effects
  • Action Potentials / physiology
  • Animals
  • Animals, Newborn
  • Efferent Pathways / cytology
  • Efferent Pathways / drug effects
  • Efferent Pathways / metabolism*
  • Mice
  • Mice, Transgenic
  • Monoamine Oxidase / deficiency
  • Monoamine Oxidase / genetics*
  • Motor Neurons / cytology
  • Motor Neurons / drug effects
  • Motor Neurons / metabolism*
  • Phrenic Nerve / cytology
  • Phrenic Nerve / drug effects
  • Phrenic Nerve / metabolism*
  • Receptor, Serotonin, 5-HT1B
  • Receptor, Serotonin, 5-HT2A
  • Receptors, Serotonin / metabolism*
  • Respiratory Center / cytology
  • Respiratory Center / drug effects
  • Respiratory Center / metabolism*
  • Respiratory Physiological Phenomena
  • Serotonin / metabolism*
  • Serotonin / pharmacology
  • Serotonin Antagonists / pharmacology
  • Serotonin Receptor Agonists / pharmacology
  • Spinal Cord / cytology
  • Spinal Cord / drug effects
  • Spinal Cord / metabolism*
  • Up-Regulation / genetics

Substances

  • Receptor, Serotonin, 5-HT1B
  • Receptor, Serotonin, 5-HT2A
  • Receptors, Serotonin
  • Serotonin Antagonists
  • Serotonin Receptor Agonists
  • Serotonin
  • Monoamine Oxidase