Abstract
In brainstem-spinal cord preparations of neonatal control C3H and transgenic Tg8 mice where deletion of the gene encoding monoamine oxidase-A results in serotonin (5-hydroxytryptamine (HT)) excess, whole cell recordings of identified phrenic motoneurons (Phr Mns) were performed to study the modulation of their activity by 5-HT. In C3H mice, a dual effect was observed: (i) a facilitation via 5-HT(2A) receptors and (ii) a decrease of the transmission of the central inspiratory drive via 5-HT(1B) receptors. In Tg8 mice, the 5-HT(2A)-mediated facilitation was present but the 5-HT(1B)-mediated decrease was lacking. Therefore, the conservation of the 5-HT(2A) response vs. the loss of the 5-HT(1B) one suggest that the two types of receptors respond differently to 5-HT level changes.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Action Potentials / drug effects
-
Action Potentials / physiology
-
Animals
-
Animals, Newborn
-
Efferent Pathways / cytology
-
Efferent Pathways / drug effects
-
Efferent Pathways / metabolism*
-
Mice
-
Mice, Transgenic
-
Monoamine Oxidase / deficiency
-
Monoamine Oxidase / genetics*
-
Motor Neurons / cytology
-
Motor Neurons / drug effects
-
Motor Neurons / metabolism*
-
Phrenic Nerve / cytology
-
Phrenic Nerve / drug effects
-
Phrenic Nerve / metabolism*
-
Receptor, Serotonin, 5-HT1B
-
Receptor, Serotonin, 5-HT2A
-
Receptors, Serotonin / metabolism*
-
Respiratory Center / cytology
-
Respiratory Center / drug effects
-
Respiratory Center / metabolism*
-
Respiratory Physiological Phenomena
-
Serotonin / metabolism*
-
Serotonin / pharmacology
-
Serotonin Antagonists / pharmacology
-
Serotonin Receptor Agonists / pharmacology
-
Spinal Cord / cytology
-
Spinal Cord / drug effects
-
Spinal Cord / metabolism*
-
Up-Regulation / genetics
Substances
-
Receptor, Serotonin, 5-HT1B
-
Receptor, Serotonin, 5-HT2A
-
Receptors, Serotonin
-
Serotonin Antagonists
-
Serotonin Receptor Agonists
-
Serotonin
-
Monoamine Oxidase