Cloning of a human tRNA isopentenyl transferase

Gene. 2000 Nov 27;258(1-2):85-93. doi: 10.1016/s0378-1119(00)00421-2.


A cDNA of human origin is shown to encode a tRNA isopentenyl transferase (E.C. Expression of the gene in a Saccharomyces cerevisiae mutant lacking the endogenous tRNA isopentenyl transferase MOD5 resulted in functional complementation and reintroduction of isopentenyladenosine into tRNA. The deduced amino acid sequence contains a number of regions conserved in known tRNA isopentenyl transferases. The similarity to the S. cerevisiae MOD5 protein is 53%, and to the Escherichia coli MiaA protein 47%. The human sequence was found to contain a single C2H2 Zn-finger-like motif, which was detected also in the MOD5 protein, and several putative tRNA transferases located by BLAST searches, but not in prokaryotic homologues.

MeSH terms

  • Alkyl and Aryl Transferases / genetics*
  • Alkyl and Aryl Transferases / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Genetic Complementation Test
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Saccharomyces cerevisiae / genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Alkyl and Aryl Transferases
  • TRIT1 protein, human
  • tRNA isopentenyltransferase

Associated data

  • GENBANK/AF074918