PEA3 and AP-1 are required for constitutive IL-8 gene expression in hepatoma cells

Biochem Biophys Res Commun. 2000 Dec 9;279(1):166-71. doi: 10.1006/bbrc.2000.3925.


Interleukin-8 (IL-8) mRNA was constitutively expressed in human hepatoma cell line, HepG2 and in human hepatocellular carcinoma (HCC), which often form hypervascular tumors. The sequence 5'-AGGAAG-3' at -137 to -132 bp of IL-8 promoter was shown to be polyomavirus enhancer A binding protein-3 (PEA3) binding site, which can cooperate with activator protein-1 (AP-1). Both PEA3 and AP-1 are essential for constitutive IL-8 expression in HepG2 cells, determined by promoter assays. Moreover, PEA3 and IL-8 proteins coexisted in HCC tissues, but not in uninvolved liver tissues. It is possible PEA3 may have important roles in tumor progression and in angiogenesis in HCC.

MeSH terms

  • Base Sequence
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology
  • Cell Line
  • DNA Primers
  • Gene Expression Regulation, Neoplastic / physiology*
  • Humans
  • Immunohistochemistry
  • Interleukin-8 / genetics*
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / pathology
  • Transcription Factor AP-1 / physiology*
  • Transcription Factors / physiology*


  • DNA Primers
  • Interleukin-8
  • Transcription Factor AP-1
  • Transcription Factors
  • transcription factor PEA3