Influenza Virus (A/HK/156/97) Hemagglutinin Expressed by an Alphavirus Replicon System Protects Chickens Against Lethal Infection With Hong Kong-origin H5N1 Viruses

Virology. 2000 Dec 5;278(1):55-9. doi: 10.1006/viro.2000.0635.


Venezuelan equine encephalitis virus replicon particles (VRP) containing the gene expressing hemagglutinin (HA) from the human Hong Kong Influenza A isolate (A/HK/156/97) were evaluated as vaccines in chicken embryos and young chicks. Expressed HA was readily detected in bird-tissue staining with anti-H5 HA antibody and in chicken cells infected with the replicon preparations following immunoprecipitation with monoclonal antibody. Birds challenged with a dose of the lethal parent virus were protected to different extents depending on the age of the bird. In ovo and 1-day-old inoculated animals that received no boost with the VRP were partially protected; birds 2 weeks of age were completely protected with a single dose of VRP.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Age Factors
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Viral / analysis
  • Antigens, Viral / genetics*
  • Antigens, Viral / immunology
  • Cells, Cultured
  • Chick Embryo
  • Chickens
  • Encephalitis Virus, Venezuelan Equine / genetics*
  • Genetic Vectors
  • Hemagglutinin Glycoproteins, Influenza Virus / analysis
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics*
  • Hemagglutinin Glycoproteins, Influenza Virus / immunology
  • Humans
  • Immunohistochemistry
  • Influenza A Virus, H5N1 Subtype*
  • Influenza A virus / immunology*
  • Influenza, Human / prevention & control*
  • Influenza, Human / veterinary
  • Influenza, Human / virology
  • Poultry Diseases / prevention & control
  • Replicon / genetics*
  • Vaccination
  • Vaccines, Synthetic / administration & dosage*
  • Viral Vaccines / administration & dosage*


  • Antibodies, Monoclonal
  • Antigens, Viral
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Vaccines, Synthetic
  • Viral Vaccines