Disparate spinal and supraspinal opioid antinociceptive responses in beta-endorphin-deficient mutant mice

Neuroscience. 2000;101(3):709-17. doi: 10.1016/s0306-4522(00)00422-x.


The role of endogenous opioid systems in the analgesic response to exogenous opiates remains controversial. We previously reported that mice lacking the peptide neurotransmitter beta-endorphin, although unable to produce opioid-mediated stress-induced antinociception, nevertheless displayed intact antinociception after systemic administration of the exogenous opiate morphine. Morphine administered by a peripheral route can activate opioid receptors in both the spinal cord and brain. However, beta-endorphin neuronal projections are confined predominantly to supraspinal nociceptive nuclei. Therefore, we questioned whether the absence of beta-endorphin would differentially affect antinociceptive responses depending on the route of opiate administration. Time- and dose-response curves were obtained in beta-endorphin-deficient and matched wild-type C57BL/6 congenic control mice using the tail-immersion/withdrawal assay. Null mutant mice were found to be more sensitive to supraspinal (i.c.v.) injection of the micro-opioid receptor-selective agonists, morphine and D-Ala(2)-MePhe(4)-Gly-ol(5) enkephalin. In contrast, the mutant mice were less sensitive to spinal (i.t.) injection of these same drugs. Quantitative receptor autoradiography revealed no differences between genotypes in the density of mu, delta, or kappa opioid receptor binding sites in either the spinal cord or pain-relevant supraspinal areas. Thus we report that the absence of a putative endogenous ligand for the mu-opioid receptor results in opposite changes in morphine sensitivity between discrete areas of the nervous system, which are not simply caused by changes in opioid receptor expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analgesics, Opioid / pharmacology*
  • Animals
  • Brain / cytology
  • Brain / drug effects*
  • Brain / metabolism
  • Dose-Response Relationship, Drug
  • Efferent Pathways / cytology
  • Efferent Pathways / drug effects*
  • Efferent Pathways / metabolism
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / pharmacology
  • Mice
  • Mice, Inbred C57BL / anatomy & histology
  • Mice, Inbred C57BL / genetics
  • Mice, Inbred C57BL / metabolism
  • Mice, Knockout
  • Morphine / pharmacology
  • Neurons / cytology
  • Neurons / drug effects*
  • Neurons / metabolism
  • Nociceptors / cytology
  • Nociceptors / drug effects*
  • Nociceptors / metabolism
  • Pain Measurement / drug effects
  • Pain Threshold / drug effects
  • Pain Threshold / physiology
  • Radioligand Assay / statistics & numerical data
  • Spinal Cord / cytology
  • Spinal Cord / drug effects*
  • Spinal Cord / metabolism
  • beta-Endorphin / deficiency*
  • beta-Endorphin / genetics


  • Analgesics, Opioid
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
  • beta-Endorphin
  • Morphine