Recent advances in the protocols of transgenic mouse mutation assays

Mutat Res. 2000 Nov 20;455(1-2):191-215. doi: 10.1016/s0027-5107(00)00077-4.


Transgenic mutation assays were developed to detect gene mutations in multiple organs of mice or rats. The assays permit (1) quantitative measurements of mutation frequencies in all tissues/organs including germ cells and (2) molecular analysis of induced and spontaneous mutations by DNA sequencing analysis. The protocols of recently developed selections in the lambda phage-based transgenic mutation assays, i.e. cII, Spi(-) and 6-thioguanine selections, are described, and a data set of transgenic mutation assays, including those using Big Blue and Muta Mouse, is presented.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Transformation, Neoplastic / genetics
  • Mice
  • Mice, Transgenic*
  • Mutagenesis
  • Mutagenicity Tests / methods*