The class IV semaphorin CD100 plays nonredundant roles in the immune system: defective B and T cell activation in CD100-deficient mice

Immunity. 2000 Nov;13(5):633-42. doi: 10.1016/s1074-7613(00)00063-7.


The class IV semaphorin CD100/Sema4D differentially utilizes two distinct receptors: plexin-B1 in nonlymphoid tissues, such as brain and kidney, and CD72 in lymphoid tissues. We have generated CD100-deficient mice and demonstrated that they have functional defects in their immune system, without apparent abnormalities in other tissues. The number of CD5(+) B-1 cells was considerably decreased in the mutant mice, whereas conventional B cells and T cells appeared to develop normally. In vitro proliferative responses and immunoglobulin production were reduced in CD100-deficient B cells. The humoral immune response against a T cell-dependent antigen and in vivo priming of T cells were also defective in the mutant mice. These results demonstrate nonredundant and essential roles of CD100-CD72 interactions in the immune system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD*
  • B-Lymphocytes / immunology*
  • Gene Expression Regulation / immunology
  • Immunity* / genetics
  • Lymphocyte Activation / genetics*
  • Lymphocyte Activation / immunology*
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / immunology*
  • Mice
  • Mice, Knockout
  • Semaphorins*
  • T-Lymphocytes / immunology*


  • Antigens, CD
  • CD100 antigen
  • Membrane Glycoproteins
  • Semaphorins