Sequences at the Somatic Recombination Sites of Immunoglobulin Light-Chain Genes

Nature. 1979 Jul 26;280(5720):288-94. doi: 10.1038/280288a0.

Abstract

The entire nucleotide sequence of a 1.7-kilobase embryonic DNA fragment containing five joining (J) DNA segments for mouse immunoglobulin kappa chain gene has been determined. Each J DNA segment can encode amino acid residues 96--108. Comparison of one of the five J DNA sequences with those of an embryonic variable (V) gene and a complete kappa chain gene permitted localisation of a precise recombination site. The 5'-flanking regions of J DNA segments could form an inverted stem structure with the 3'-non-coding region of embryonic V genes. This hypothetical structure and gel-blotting analysis of total embryo and myeloma DNA suggest that the somatic recombination may be accompanied by excision of an entire DNA segment between a V gene and a J DNA segment. Antibody diversity may in part be generated by modulation of the precise recombination sites.

MeSH terms

  • Animals
  • Base Sequence
  • DNA / genetics
  • Genes*
  • Genetic Code
  • Immunoglobulin Light Chains / genetics*
  • Immunoglobulin kappa-Chains / genetics
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred NZB
  • Myeloma Proteins / genetics
  • Neoplasms, Experimental
  • Recombination, Genetic*

Substances

  • Immunoglobulin Light Chains
  • Immunoglobulin kappa-Chains
  • Myeloma Proteins
  • DNA

Associated data

  • GENBANK/J00549
  • GENBANK/J00551
  • GENBANK/J00557
  • GENBANK/J00558
  • GENBANK/J00559
  • GENBANK/X00268