Abstract
Auxin plays a key role in lateral root formation, but the signaling pathway for this process is poorly understood. We show here that NAC1, a new member of the NAC family, is induced by auxin and mediates auxin signaling to promote lateral root development. NAC1 is a transcription activator consisting of an N-terminal conserved NAC-domain that binds to DNA and a C-terminal activation domain. This factor activates the expression of two downstream auxin-responsive genes, DBP and AIR3. Transgenic plants expressing sense or antisense NAC1 cDNA show an increase or reduction of lateral roots, respectively. Finally, TIR1-induced lateral root development is blocked by expression of antisense NAC1 cDNA, and NAC1 overexpression can restore lateral root formation in the auxin-response mutant tir1, indicating that NAC1 acts downstream of TIR1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis / metabolism
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Arabidopsis Proteins*
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Base Sequence
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Cotyledon / metabolism
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DNA, Complementary
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology
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Dimerization
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Gene Expression
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Indoleacetic Acids / metabolism*
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Molecular Sequence Data
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Oligonucleotides, Antisense
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Peptide Synthases / metabolism*
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Plant Leaves / metabolism
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plant Proteins / physiology
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Plant Roots / growth & development
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Plants, Genetically Modified
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Protein Structure, Tertiary
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Response Elements
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SKP Cullin F-Box Protein Ligases
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Signal Transduction / physiology*
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Trans-Activators / physiology
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Transcriptional Activation
Substances
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Arabidopsis Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Indoleacetic Acids
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NAC1 protein, Arabidopsis
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Oligonucleotides, Antisense
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Plant Proteins
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Trans-Activators
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SKP Cullin F-Box Protein Ligases
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Peptide Synthases
Associated data
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GENBANK/AC002304
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GENBANK/AF198054