Abstract
We investigated the anti-inflammatory effects of aqueous extract from Lonicera japonica flower (AELJ), a traditional skin rash drug, in lipopolysaccharide (LPS)-induced rat liver sepsis. Immunoblot analysis showed that the level of nuclear factor (NF)-kappaBp65 was rapidly up-regulated and inhibitory (I)-kappaBalpha was down-regulated by LPS challenge. However, AELJ inhibited the increase of NF-kappaBp65 and degradation of I-kappaBalpha in the liver of LPS-challenged rats. Immunohistochemical analysis of rat hepatocytes showed that LPS-induced inflammatory responses, involving degradation of I-kappaBalpha and induction of NF-kappaBp65, tumor necrosis factor (TNF)-alpha and inducible nitric oxide synthase (iNOS), are partially inhibited by pretreatment with AELJ. These results suggest that AELJ may act as a therapeutic agent for inflammatory disease through a selective regulation of NF-kappaB activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / pharmacology*
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Blotting, Western
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Hepatocytes / chemistry
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Hepatocytes / cytology
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Hepatocytes / drug effects
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I-kappa B Proteins / metabolism*
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Immunohistochemistry
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Lipopolysaccharides / pharmacology
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Liver / chemistry
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Liver / drug effects*
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Liver / metabolism
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Male
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NF-kappa B / metabolism*
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Nitric Oxide Synthase / drug effects
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Nitric Oxide Synthase / metabolism
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Nitric Oxide Synthase Type II
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plants, Medicinal / chemistry*
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Rats
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Rats, Sprague-Dawley
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Transcription Factor RelA
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Tumor Necrosis Factor-alpha / drug effects
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Anti-Inflammatory Agents
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I-kappa B Proteins
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Lipopolysaccharides
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NF-kappa B
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Plant Extracts
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Transcription Factor RelA
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Tumor Necrosis Factor-alpha
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, rat