Abstract
Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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COS Cells
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Clostridium tetani / genetics
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Clostridium tetani / immunology
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Codon / genetics*
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CpG Islands
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DNA Primers / genetics
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Female
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Gene Expression
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Genes, Bacterial
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Immunization
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Injections, Intramuscular
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Mice
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Mice, Inbred BALB C
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Peptide Fragments / genetics*
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Peptide Fragments / immunology*
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Plasmids / genetics
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Tetanus Antitoxin / blood
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Tetanus Toxin / genetics*
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Tetanus Toxin / immunology*
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Tetanus Toxoid / administration & dosage
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Tetanus Toxoid / genetics*
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Tetanus Toxoid / immunology*
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Transfection
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / genetics*
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Vaccines, DNA / immunology*
Substances
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Codon
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DNA Primers
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Peptide Fragments
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Tetanus Antitoxin
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Tetanus Toxin
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Tetanus Toxoid
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Vaccines, DNA
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tetanus toxin fragment C