The regulation of protein function by multisite phosphorylation--a 25 year update

Trends Biochem Sci. 2000 Dec;25(12):596-601. doi: 10.1016/s0968-0004(00)01712-6.


The phosphorylation of a protein can alter its behaviour in almost every conceivable way. These include modulation of its intrinsic biological activity, subcellular location, half-life and docking with other proteins. 'Multisite phosphorylation can enable several such effects to operate in the same protein. It can also determine the extent and duration of a response and is the key to signal integration.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cations / metabolism
  • Phosphorylation
  • Protein Kinases / metabolism*
  • Proteins / metabolism*
  • Signal Transduction*
  • Transcriptional Activation


  • Cations
  • Proteins
  • Protein Kinases