Immunohistochemical localization of the P2Y1 purinergic receptor in Alzheimer's disease

Neuroreport. 2000 Nov 27;11(17):3799-803. doi: 10.1097/00001756-200011270-00041.


The biological actions of extracellular nucleotides are mediated by two distinct classes of P2 receptor, P2X and P2Y. The G protein-coupled P2Y receptors comprise five mammalian subtypes, P2Y(1-11). The P2Y1 subtype is expressed abundantly throughout the human brain and is specifically localized to neuronal structures. In the present study, the distribution of the P2Y1 receptor was investigated in Alzheimer's disease (AD) brains. In contrast to control human brain, the P2Y1 receptor was localized to a number of characteristic AD structures such as neurofibrillary tangles, neuritic plaques and neuropil threads. Immunoblot analysis showed that this specific immunostaining observed over tangles was not a result of cross-reactivity between the anti-P2Y1 antiserum and abnormal tau protein, the major constituent of tangles. The significance of this altered P2Y1 cellular distribution in AD brains is at present unclear.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / metabolism
  • Adenosine Triphosphate / metabolism
  • Aged
  • Alzheimer Disease / metabolism*
  • Alzheimer Disease / pathology*
  • Brain / pathology*
  • Brain Chemistry / physiology*
  • Entorhinal Cortex / metabolism
  • Entorhinal Cortex / pathology
  • Female
  • Hippocampus / metabolism
  • Hippocampus / pathology
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Neurofibrillary Tangles / metabolism
  • Neurofibrillary Tangles / pathology
  • Plaque, Amyloid / metabolism
  • Plaque, Amyloid / pathology
  • Pyramidal Tracts / metabolism
  • Pyramidal Tracts / pathology
  • Receptors, Purinergic P2 / metabolism*
  • Receptors, Purinergic P2Y1
  • tau Proteins / metabolism


  • P2RY1 protein, human
  • Receptors, Purinergic P2
  • Receptors, Purinergic P2Y1
  • tau Proteins
  • Adenosine Diphosphate
  • Adenosine Triphosphate