Characteristics of CA1 neurons recorded intracellularly in the hippocampal in vitro slice preparation

Brain Res. 1975 Mar 7;85(3):423-36. doi: 10.1016/0006-8993(75)90817-3.


Intracellular recordings were made from CA1 pyramidal cells in the hippocampal in vitro slice preparation. Neurons displayed characteristics previously described for the intact preparation including: EPSPs, IPSPs, depolarizing after-potentials, bursting, frequency potentiation, double pulse facilitation, and probably dendritis spiking. Cells could be discharged when orthodromic or antidromic pathways were stimulated, with injection of a depolarizing current pulse, and from iontophoresis of glutamic acid. The apparently normal behavior of the neurons makes the isolated hippocampal slice a promising cortical preparation.

MeSH terms

  • Action Potentials
  • Animals
  • Electric Stimulation
  • Electrodes
  • Evoked Potentials*
  • Glutamates / pharmacology
  • Guinea Pigs
  • Hippocampus / physiology*
  • In Vitro Techniques
  • Iontophoresis
  • Membrane Potentials
  • Synapses / physiology


  • Glutamates