Kidney produces a novel acylated peptide, ghrelin

FEBS Lett. 2000 Dec 15;486(3):213-6. doi: 10.1016/s0014-5793(00)02308-5.


Ghrelin is a novel growth hormone-releasing peptide with a unique acylated structure. Here we reveal that prepro-ghrelin gene is expressed in the mouse kidney and glomerulus. We also show by reverse-phase high performance liquid chromatography coupled with radioimmunoassay that the mouse kidney does produce ghrelin. The ghrelin immunoreactivity in the mouse kidney is 6.79+/-0.48 fmol/mg (n=5), which is much more abundant than that in the mouse plasma of 0.339+/-0.029 fmol/microl (n=6). Furthermore, prepro-ghrelin gene is expressed in cultured rat mesangial cells, fibroblast-like NRK-49F cells and mouse podocytes, but not in rat epithelial cell-like NRK-52E cells. Ghrelin receptor gene is also expressed in the rat kidney. These findings demonstrate that the kidney, glomerulus and renal cells express prepro-ghrelin gene and ghrelin is produced locally in the kidney, and suggest the endocrine and/or paracrine roles of ghrelin in the kidney.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acylation
  • Animals
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Gene Expression
  • Ghrelin
  • Glomerular Mesangium / cytology
  • Glomerular Mesangium / metabolism
  • Kidney / cytology
  • Kidney / metabolism*
  • Kidney Glomerulus / cytology
  • Kidney Glomerulus / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Peptide Hormones*
  • Peptides / blood
  • Peptides / genetics
  • Peptides / metabolism*
  • Radioimmunoassay
  • Rats
  • Rats, Inbred WKY
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / genetics
  • Receptors, G-Protein-Coupled*
  • Receptors, Ghrelin
  • Reverse Transcriptase Polymerase Chain Reaction


  • Ghrelin
  • Peptide Hormones
  • Peptides
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Receptors, Ghrelin