Immune response to Plasmodium falciparum liver stage antigen-1: geographical variations within Central Africa and their relationship with protection from clinical malaria

Trans R Soc Trop Med Hyg. Sep-Oct 2000;94(5):557-62. doi: 10.1016/s0035-9203(00)90086-5.

Abstract

Two populations of schoolchildren from Gabon and Cameroon were tested in 1995 for their immunological reactivity to synthetic peptides (LSA-Rep, LSA-J and LSA-CTL) from Plasmodium falciparum liver stage antigen-1 (LSA-1). The prevalence and levels of both cellular (lymphocyte proliferation, tumour necrosis factor alpha (TNF alpha), interferon gamma (IFN gamma), and interleukin-10 (IL-10)) and humoral (immunoglobulin G) responses were determined. Protection from clinical malaria, determined after a prospective 1 year study in both sites, was associated with elevated proliferative responses to LSA-Rep and LSA-CTL in the Gabonese children, as well as with higher antibody levels to both schizont extract and LSA-Rep. The prevalence of peptide-stimulated TNF-alpha secretion was higher in the Cameroonian group, but higher levels of antibodies to LSA-Rep and LSA-J were found in the Gabonese children. The immunological differences observed between children in the 2 study sites are discussed in the context of both epidemiological and individual host factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Formation
  • Antigens, Protozoan / immunology*
  • Cameroon
  • Child
  • Cohort Studies
  • Cytokines / immunology
  • Female
  • Gabon
  • Humans
  • Leukocytes, Mononuclear / immunology
  • Liver Diseases, Parasitic / immunology*
  • Lymphocyte Activation
  • Malaria, Falciparum / immunology*
  • Male
  • Plasmodium falciparum / immunology*

Substances

  • Antigens, Protozoan
  • Cytokines