Folate deficiency reduces the development of colorectal cancer in rats

Carcinogenesis. 2000 Dec;21(12):2261-5. doi: 10.1093/carcin/21.12.2261.


Alterations in folate status may play an important role in carcinogenesis. The aim of this study was to examine the effect of a diminished folate status on azoxymethane (AOM)-induced intestinal tumours in Sprague-Dawley rats. A total of 125 weanling male rats were divided into five equal groups and fed semi-purified diets containing either 8 mg/kg folate or no folate. After 4 weeks on experimental diets, all animals received three weekly subcutaneous injections of AOM at a dose rate of 15 mg/kg bodyweight. The animals were necropsied after 26 weeks. Rats with a diminished folate status, evident by significantly reduced blood and colonic folate concentrations and elevated plasma homocysteine levels, had significantly (P < 0.01) lower incidence and number of small intestinal and colonic tumours compared with rats displaying an adequate folate status. There was a significant decrease in the incidence of colonic adenocarcinomas (P < 0.01) and size of colonic tumours observed in the rats displaying a diminished folate status. This study shows that a diminished folate status was associated with a decrease in the development of AOM-induced colorectal cancers. The decrease in risk may be attributed to the known role of folate in cell multiplication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / chemically induced
  • Adenocarcinoma / pathology
  • Adenocarcinoma / prevention & control
  • Adenoma / chemically induced
  • Adenoma / pathology
  • Adenoma / prevention & control
  • Animals
  • Azoxymethane
  • Colonic Neoplasms / chemically induced*
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / prevention & control
  • Colorectal Neoplasms / chemically induced*
  • Colorectal Neoplasms / pathology
  • Colorectal Neoplasms / prevention & control
  • Folic Acid / blood
  • Folic Acid / pharmacokinetics
  • Folic Acid / pharmacology*
  • Folic Acid Deficiency / physiopathology*
  • Intestinal Neoplasms / chemically induced*
  • Intestinal Neoplasms / pathology
  • Intestinal Neoplasms / prevention & control
  • Male
  • Rats
  • Rats, Sprague-Dawley


  • Folic Acid
  • Azoxymethane