Gradient Formation of the TGF-beta Homolog Dpp

Cell. 2000 Dec 8;103(6):981-91. doi: 10.1016/s0092-8674(00)00200-2.

Abstract

Secreted morphogens such as the Drosophila TGF-beta homolog Decapentaplegic (Dpp) are thought to spread through target tissues and form long-range concentration gradients providing positional information. Using a GFP-Dpp fusion, we monitored a TGF-beta family member trafficking in situ throughout the target tissue and forming a long-range concentration gradient. Evidence is presented that long-range Dpp movement involves Dpp receptor and Dynamin functions. We also show that the rates of endocytic trafficking and degradation determine Dpp signaling range. We propose a model where the gradient is formed via intracellular trafficking initiated by receptor-mediated endocytosis of the ligand in receiving cells with the gradient slope controlled by endocytic sorting of Dpp toward recycling versus degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Body Patterning*
  • Drosophila Proteins*
  • Drosophila melanogaster / anatomy & histology
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / physiology*
  • Dynamins
  • Endocytosis
  • Endosomes / metabolism
  • GTP Phosphohydrolases / genetics
  • GTP Phosphohydrolases / metabolism
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Immunohistochemistry
  • Insect Proteins / genetics*
  • Insect Proteins / metabolism*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Protein Transport* / physiology
  • Recombinant Fusion Proteins / metabolism
  • Temperature
  • Transforming Growth Factor beta / chemistry
  • Transforming Growth Factor beta / metabolism*
  • Wings, Animal / anatomy & histology
  • Wings, Animal / physiology
  • rab GTP-Binding Proteins / genetics
  • rab GTP-Binding Proteins / metabolism

Substances

  • Drosophila Proteins
  • Insect Proteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Transforming Growth Factor beta
  • dpp protein, Drosophila
  • Green Fluorescent Proteins
  • GTP Phosphohydrolases
  • rab GTP-Binding Proteins
  • Dynamins