Xanthine oxidase-derived reactive oxygen species activate nuclear factor kappa B during hepatic ischemia in rats

Jpn J Pharmacol. 2000 Nov;84(3):363-6. doi: 10.1254/jjp.84.363.

Abstract

The present study was carried out to elucidate the relationship between the generation of reactive oxygen species (ROS) and an activation of nuclear factor (NF) kappa B in a hepatic ischemia-reperfusion model. During the ischemic period, the contents of xanthine oxidase (XOD) metabolites and thiobarbituric acid-reactive substances were significantly increased, and NF-kappa B was activated in the liver of rats. The activation of NF-kappa B was inhibited by pretreatment of allopurinol (10-100 mg/kg, i.p.) in a dose-dependent manner. In conclusion, this suggests that the XOD-derived ROS may activate NF-kappa B during ischemia.

MeSH terms

  • Animals
  • Ischemia / metabolism*
  • Liver / blood supply*
  • Male
  • NF-kappa B / metabolism*
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species / metabolism*
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Xanthine Oxidase / metabolism*

Substances

  • NF-kappa B
  • Reactive Oxygen Species
  • Thiobarbituric Acid Reactive Substances
  • Xanthine Oxidase