Direct Proof for the Presence and Expression of Two 5% Different 16S rRNA Genes in Individual Cells of Haloarcula Marismortui

Extremophiles. 2000 Dec;4(6):373-6. doi: 10.1007/s007920070007.

Abstract

A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers
  • Haloarcula marismortui / genetics*
  • In Situ Hybridization, Fluorescence
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics*

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S