New structural and chemical insight into the catalytic mechanism of epoxide hydrolases

Drug Metab Rev. Aug-Nov 2000;32(3-4):327-38. doi: 10.1081/dmr-100102337.

Abstract

New structural and mechanistic data on epoxide hydrolases provide additional insight into the details of how these enzymes function. The data reveal that, in addition to the catalytic triad located in the core domain that carries out the hydrolytic reaction, there are two tyrosine residues, located in the cap domain, which assist in the initial alkylation event.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Binding, Competitive
  • Catalysis
  • Epoxide Hydrolases / chemistry*
  • Epoxide Hydrolases / metabolism*
  • Epoxy Compounds / metabolism
  • Forecasting
  • Humans
  • Hydrolysis
  • Kinetics
  • Models, Molecular
  • Protein Conformation

Substances

  • Epoxy Compounds
  • Epoxide Hydrolases