Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae

Can J Microbiol. 2000 Dec;46(12):1096-100. doi: 10.1139/w00-105.

Abstract

Cytosolic glycerol kinase (Gut1p) and mitochondrial glycerol-3-phosphate dehydrogenase (Gut2p) constitute the glycerol utilization pathway in Saccharomyces cerevisiae. Transcriptional analysis of the GUT2 gene showed that it was repressed by glucose and derepressed on the non-fermentable carbon sources, glycerol, lactate and ethanol. Derepression of GUT2 requires the protein kinase Snflp as well as the heteromeric protein complex, Hap2/3/4/5, and its putative DNA-binding site (UASHAP) located in the promoter region. Furthermore, glucose repression of GUT2 requires the negative regulator, Opi1p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CCAAT-Binding Factor / metabolism
  • Ethanol / metabolism
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal
  • Glucose / metabolism
  • Glycerol / metabolism
  • Glycerolphosphate Dehydrogenase / biosynthesis
  • Glycerolphosphate Dehydrogenase / genetics*
  • Lactic Acid / metabolism
  • Mitochondria / enzymology*
  • Promoter Regions, Genetic
  • Protein-Serine-Threonine Kinases / metabolism
  • Regulatory Sequences, Nucleic Acid
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / metabolism
  • Transcription, Genetic*
  • Transformation, Genetic

Substances

  • CCAAT-Binding Factor
  • Fungal Proteins
  • HAP3 protein, S cerevisiae
  • OPI1 protein, S cerevisiae
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Lactic Acid
  • Ethanol
  • Glycerolphosphate Dehydrogenase
  • SNF1-related protein kinases
  • Protein-Serine-Threonine Kinases
  • Glucose
  • Glycerol