Similarity of antibiotic resistance patterns and molecular typing properties of methicillin-resistant Staphylococcus aureus isolates widely spread in hospitals in New York City and in a hospital in Tokyo, Japan

Microb Drug Resist. Fall 2000;6(3):253-8. doi: 10.1089/mdr.2000.6.253.

Abstract

One hundred and forty-three single-patient methicillin-resistant Staphylococcus aureus (MRSA) isolates collected during April-June, 1997, and February, 1998, in a hospital in Tokyo, Japan, were characterized by molecular typing techniques that involved hybridization of ClaI restriction digests with the mecA- and Tn554-specific DNA probes and determination of macrorestriction patterns of SmaI-digested chromosomal DNA by pulsed-field gel electrophoresis (PFGE). A large proportion (76%) of the isolates carried the mecA polymorph I, Tn554 pattern A, and PFGE pattern A (clonal type I:A:A), which was the same as the clonal type of an MRSA widely spread in hospitals in New York City and hospitals in neighboring New Jersey, Connecticut, and Pennsylvania. Also similarly to the New York clone, most of the MRSA isolates from the Japanese hospital were resistant to penicillin, ciprofloxacin, erythromycin, tetracycline, and high concentrations (500 microg/ml) of spectinomycin, but were susceptible to chloramphenicol, sulfamethoxazole-trimethoprim, and rifampin. All of the 143 MRSA isolates had vancomycin MICs < or = 2 mg/L.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins*
  • Bacterial Typing Techniques
  • Carrier Proteins / genetics
  • Clone Cells
  • DNA Transposable Elements / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Female
  • Hexosyltransferases*
  • Hospitals, Urban*
  • Humans
  • Male
  • Methicillin Resistance / genetics*
  • Microbial Sensitivity Tests
  • Muramoylpentapeptide Carboxypeptidase / genetics
  • New York City / epidemiology
  • Penicillin-Binding Proteins
  • Peptidyl Transferases*
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / genetics
  • Tokyo / epidemiology
  • Vancomycin / pharmacology

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carrier Proteins
  • DNA Transposable Elements
  • Penicillin-Binding Proteins
  • Vancomycin
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase