Involvement of transglutaminase in the receptor-mediated endocytosis of mouse peritoneal macrophages

Biol Pharm Bull. 2000 Dec;23(12):1511-3. doi: 10.1248/bpb.23.1511.


We investigated the effect of extracellular Ca2+ on the uptake of immunoglobulin G (IgG)-coated normal sheep red blood cells (SRBC) by the mouse peritoneal macrophages. The uptake of SRBC was dose dependently enhanced by Ca2+ concentration. We also investigated the effect of ryanodine on the uptake of SRBC. Ryanodine showed approximately 15% inhibition of the uptake. The activity of transglutaminase (TGase) in the cytosolic fraction of macrophages was enhanced as the Ca2+ concentration increased. The uptake of SRBC decreased by the addition of histamine, a TGase inhibitor. These results suggest that TGase is involved in the receptor-mediated endocytosis system of mouse peritoneal macrophages.

MeSH terms

  • Animals
  • Calcium / pharmacology
  • Endocytosis / drug effects
  • Endocytosis / physiology*
  • Erythrocytes / immunology
  • Erythrocytes / metabolism
  • Histamine / pharmacology
  • Immunoglobulin G / immunology
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / enzymology
  • Macrophages, Peritoneal / immunology
  • Macrophages, Peritoneal / physiology*
  • Male
  • Mice
  • Mice, Inbred C3H
  • Receptors, Fc / physiology*
  • Sheep
  • Transglutaminases / antagonists & inhibitors
  • Transglutaminases / metabolism*


  • Immunoglobulin G
  • Receptors, Fc
  • Histamine
  • Transglutaminases
  • Calcium