Potential contribution of IL-17-producing Th(1)cells to defective repair activity in joint inflammation: partial correction with Th(2)-promoting conditions

Cytokine. 2001 Jan 21;13(2):113-8. doi: 10.1006/cyto.2000.0811.

Abstract

To assess the contribution of cell interactions to the production of cytokines and type I collagen, fixed synovium T cell clones were cocultured on synoviocytes and levels of IL-6, LIF and PICP, a marker of type I collagen synthesis measured. Levels of IL-6 and LIF were higher with Th(1)than with Th(0)and Th(2)clones. Levels of PICP were decreased with Th(1)clones and increased with Th(2)clones. IL-17-producing T cells, all Th(1), were among the highest inducers of cytokine and inhibitors of collagen synthesis. Preincubation of clones in Th(1)conditions (IL-12 plus anti-IL-4) increased IL-6 production, whereas Th(2)conditions (IL-4 plus anti-IL-12) strongly inhibited IL-6 production and restored repair activity. As rheumatoid synovium is infiltrated by Th(1)cells, local cell interactions result in a pro-inflammatory pattern with defective repair, which can be reversed at least in part, by a Th(2)pattern.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid / metabolism
  • Coculture Techniques
  • Collagen / biosynthesis
  • Culture Media / metabolism
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Growth Inhibitors / biosynthesis
  • Humans
  • Inflammation*
  • Interleukin-12 / pharmacology
  • Interleukin-17 / biosynthesis*
  • Interleukin-4 / pharmacology
  • Interleukin-6 / biosynthesis
  • Joints / metabolism*
  • Leukemia Inhibitory Factor
  • Lymphokines / biosynthesis
  • Synovial Fluid / cytology*
  • Th1 Cells / metabolism*
  • Th2 Cells / metabolism*
  • Time Factors

Substances

  • Culture Media
  • Growth Inhibitors
  • Interleukin-17
  • Interleukin-6
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lymphokines
  • Interleukin-12
  • Interleukin-4
  • Collagen