Molecular analysis of the mitotic checkpoint genes BUB1, BUBR1 and BUB3 in human lung cancers

Cancer Lett. 2001 Jan 26;162(2):201-5. doi: 10.1016/s0304-3835(00)00675-3.

Abstract

Our previous studies showed that mitotic checkpoint impairment is present in about 40% of human lung cancer cell lines but that mutations in the MAD mitotic checkpoint genes are infrequent. In the present study, we examined 44 lung cancer cases for the potential involvement of the other gene family involved in the mitotic checkpoint, i.e. BUB. We found that the BUB gene family members including BUB1, BUBR1 and BUB3 are not frequent targets for mitotic checkpoint defects in lung cancers, if present at all. Further studies are thus warranted to elucidate the molecular basis for the acquisition of mitotic checkpoint defects in order to better understand the molecular pathogenesis of lung cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / genetics*
  • DNA Mutational Analysis
  • Humans
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Mitosis / genetics*
  • Poly-ADP-Ribose Binding Proteins
  • Polymorphism, Single-Stranded Conformational
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases
  • Proteins / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • BUB3 protein, human
  • Cell Cycle Proteins
  • Poly-ADP-Ribose Binding Proteins
  • Proteins
  • Protein Kinases
  • BUB1 protein, human
  • Bub1 spindle checkpoint protein
  • Protein-Serine-Threonine Kinases