Quantifying tracer levels of (2)H(2)O enrichment from microliter amounts of plasma and urine by (2)H NMR

Magn Reson Med. 2001 Jan;45(1):156-8. doi: 10.1002/1522-2594(200101)45:1<156::aid-mrm1020>3.0.co;2-z.


A simple and sensitive (2)H NMR measurement of (2)H(2)O enrichment from a 10 microl volume of body fluid is presented. The method allows (2)H-enrichment levels of 0.1% or above to be rapidly determined from 10 microl of plasma or urine. The measurement is insensitive to the presence of plasma protein, allowing direct analysis of (2)H(2)O enrichment from native plasma samples. Magn Reson Med 45:156-158, 2001.

MeSH terms

  • Animals
  • Deuterium Oxide / blood*
  • Deuterium Oxide / urine*
  • Humans
  • Magnetic Resonance Spectroscopy / methods*
  • Rats


  • Deuterium Oxide