Multi-step control of spindle pole body duplication by cyclin-dependent kinase

Nat Cell Biol. 2001 Jan;3(1):38-42. doi: 10.1038/35050543.


Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Cycle / genetics
  • Cell Transformation, Neoplastic / genetics*
  • Centrosome / enzymology*
  • Cyclin B / genetics
  • Cyclin B / metabolism
  • Cyclin-Dependent Kinases / genetics
  • Cyclin-Dependent Kinases / metabolism*
  • Cyclins / genetics
  • Cyclins / metabolism*
  • Mitosis / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Spindle Apparatus / enzymology*
  • Yeasts / genetics*
  • Yeasts / metabolism


  • CLB5 protein, S cerevisiae
  • CLB6 protein, S cerevisiae
  • Cyclin B
  • Cyclins
  • Saccharomyces cerevisiae Proteins
  • Cyclin-Dependent Kinases