The synaptic vesicle protein, cysteine-string protein, is associated with the plasma membrane in 3T3-L1 adipocytes and interacts with syntaxin 4

J Cell Sci. 2001 Jan;114(Pt 2):445-55.

Abstract

Adipocytes and muscle cells play a major role in blood glucose homeostasis. This is dependent upon the expression of Glut4, an insulin-responsive facilitative glucose transporter. Glut4 is localised to specialised intracellular vesicles that fuse with the plasma membrane in response to insulin stimulation. The insulin-induced translocation of Glut4 to the cell surface is essential for the maintenance of optimal blood glucose levels, and defects in this system are associated with insulin resistance and type II diabetes. Therefore, a major focus of recent research has been to identify and characterise proteins that regulate Glut4 translocation. Cysteine-string protein (Csp) is a secretory vesicle protein that functions in presynaptic neurotransmission and also in regulated exocytosis from non-neuronal cells. We show that Csp1 is expressed in 3T3-L1 adipocytes and that cellular levels of this protein are increased following cell differentiation. Combined fractionation and immunofluorescence analyses reveal that Csp1 is not a component of intracellular Glut4-storage vesicles (GSVs), but is associated with the adipocyte plasma membrane. This association is stable, and not affected by either insulin stimulation or chemical depalmitoylation of Csp1. We also demonstrate that Csp1 interacts with the t-SNARE syntaxin 4. As syntaxin 4 is an important mediator of insulin-stimulated GSV fusion with the plasma membrane, this suggests that Csp1 may play a regulatory role in this process. Syntaxin 4 interacts specifically with Csp1, but not with Csp2. In contrast, syntaxin 1A binds to both Csp isoforms, and actually exhibits a higher affinity for the Csp2 protein. The results described raise a number of interesting questions concerning the intracellular targeting of Csp in different cell types, and suggest that the composition and synthesis of GSVs may be different from synaptic and other secretory vesicles. In addition, the interaction of Csp1 with syntaxin 4 suggests that this Csp isoform may play a role in insulin-stimulated fusion of GSVs with the plasma membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipocytes / physiology*
  • Animals
  • Brain / metabolism
  • Cell Fractionation
  • Cell Membrane / metabolism*
  • Cell Membrane / ultrastructure
  • Glucose Transporter Type 4
  • HSP40 Heat-Shock Proteins
  • Insulin / pharmacology
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Monosaccharide Transport Proteins / metabolism
  • Muscle Proteins*
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / metabolism
  • Organelles / physiology
  • Organelles / ultrastructure
  • Protein Transport
  • Qa-SNARE Proteins
  • Recombinant Fusion Proteins / biosynthesis
  • SNARE Proteins
  • Synaptic Vesicles / physiology
  • Syntaxin 1
  • Transfection
  • Triiodobenzoic Acids
  • Vesicular Transport Proteins*

Substances

  • Glucose Transporter Type 4
  • HSP40 Heat-Shock Proteins
  • Insulin
  • Membrane Proteins
  • Monosaccharide Transport Proteins
  • Muscle Proteins
  • Nerve Tissue Proteins
  • Qa-SNARE Proteins
  • Recombinant Fusion Proteins
  • SNARE Proteins
  • Slc2a4 protein, mouse
  • Stx1a protein, mouse
  • Syntaxin 1
  • Triiodobenzoic Acids
  • Vesicular Transport Proteins
  • cysteine string protein
  • iodixanol